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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in selling mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.

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Blocking peptides are peptides that bind precisely into the concentrate on antibody and block antibody binding. These peptide normally includes the epitope identified by the antibody.

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This guide outlines further more feasible optimization of mobile and immunoassay parameters to make sure the best possible results are...

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This manual outlines further feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies bound to the blocking peptide now not bind for the epitope about the target protein. By comparing the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded here as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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